Beads are composed of a murine derived, anti-FLAG ® clone M2 monoclonal antibody attached to superparamagnetic iron impregnated, 4% agarose beads. A FLAG tag can be used in many different assays that require recognition by an antibody. FLAG tags are also easily removed by enterokinase (EK).
Binding is calcium-independent. Not for use in diagnostic procedures. The FLAG tag may be inserted at the N terminus, the N terminus preceded by a methionine residue, the C terminus, or internal positions of the target protein.
Some commercially available antibodies (e.g., M1/4E11) recognize the The first use of epitope tagging was described by Munro and Pelham in 1984.The third report of epitope tagging, ( Fluorescent and enzyme-conjugated versions of our FLAG Tag antibodies are available for your added convenience.For Research Use Only. 1:500-1:5000 *The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user. Anti-FLAG ® M2 Magnetic Beads for FLAG ® Tag Protein Capture. M1 M1 anti-FLAG ® antibody binding is calcium-dependent and is specific for the N-terminus of the FLAG ® tag. Deep Discount Custom Antibody ProductionMouse IgG monoclonal antibody for Flag Tag detection. Not for use in diagnostic procedures. IP antibody use£º5ug Flag Mouse IgG1 per ml Lysate£¬WB 1: 5000 1 ) untransfected 293 cell lysate 2 ) transfected 293 cell lysate with Flag-tag fusion protein 3 ) IP (transfected 293+ normal Mouse IgG+Protein G agarose) 4 ) IP (transfected 293+anti- Flag mAb+ Protein G agarose) 5 ) IP (transfected 293+Protein G) 6 ) Recombinant protein (E.coli). The FLAG tag (peptide sequence DYKDDDDK) is a short, hydrophilic protein tag commonly used in conjunction with antibodies in protein pull-downs to study protein–protein interactions.
FLAG tags are also easily removed by enterokinase (EK). Method of purification - Protein A Immunogen FLAG; … Because of its hydrophilic nature, the FLAG tag is commonly found on the surface of a fusion protein, which makes it more available as an epitope for binding to antibodies.
To order the blocking peptide or the immungen protein ($99 / 1mg) for Anti-Flag Tag Antibody (monoclonal, 1E6) M30971, contact Did you know Boster offer custom antibody production service? FLAG and 3xFLAG are useful in Western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the …
It can be fused to the C-terminus or the N-terminus of a protein, or inserted within a protein. The FLAG tag (peptide sequence DYKDDDDK) is a short, hydrophilic protein tag commonly used in conjunction with antibodies in protein pull-downs to study protein–protein interactions. Our Boster Quality Guarantee for Anti-Flag Tag Antibody (monoclonal, 1E6) covers its use in the following applications. Anti-FLAG ® M2 Magnetic Beads for FLAG ® Tag Protein Capture.