Guarantee. Glycoaldehyde inactivates GAPD. Please contact us to place your order, or try again later. This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation. Positive Tested Species Reactivity: Human. Positive Controls: c4 whole cell lysate: sc-364186, Jurkat whole cell lysate: sc-2204 or MOLT-4 cell lysate: sc-2233. representative of CST, are rejected and are of no force or effect. Preliminary data indicates that the GAPDH antibody- loading control ab8245 recognizes the monomer (36 kDa) and also the dimer forms of GAPDH, but not the tetrameric form of the protein. Membrane was incubated with anti-GAPDH in blocking buffer (1:5000) at 4C overnight. Aspirate media from cultures; wash cells with 1X PBS; aspirate. Molecular weight (kD):30-40 Because the GAPDH gene is often stably and constitutively expressed at high levels in most tissues and cells, it is considered a housekeeping gene. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). antibody diluted in 5%milk. Access advice and support for any research roadblock, Full event breakdown with abstracts, speakers, registration and more, Supporting our customers and employees during the COVID-19 pandemic. Federica (Verified Customer) (06-08-2020). Calculated molecular weight: 36 kDa: Observed molecular weight: 36 kDa: GenBank accession number: BC004109: Gene symbol: GAPDH: Gene ID (NCBI) 2597: Conjugate: Unconjugated: Form: Liquid: Purification Method: Antigen affinity purification: Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. The molecular weight of RFP (calculated) is 27 kDa, but in a gel it is expected to run at approximately 28-30 kDa. Wash membrane-bound HRP (antibody conjugate) three times for 5 minutes in TBST. Excellent antibody.I used in WB on HeLa lysate and the results was extremely clear. Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively (PubMed:3170585, PubMed:11724794). Various lysates were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Would you like to visit your country specific website? Incubate for 30 min at room temperature. Trademark Information. Discard supernatant in appropriate GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. What are the known protein-protein interactions of GAPDH? RNA was isolated from 80S (monosomes), low-molecular-weight and high-molecular-weight fractions. (Generally, 5x10. NOTE: When including fluorescent cellular dyes in your experiment (including viability dyes, DNA dyes, etc. 1/1000. HRP-60004 targets GAPDH in WB applications and shows reactivity with human, mouse, rat, zebrafish, plant samples. Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. Actin filaments were labeled with DY-554 phalloidin (red). Found inside – Page 362285 - 3.2 kb - 2.5 kb 18S - MMP - 9 GAPDH Only low - molecular - weight HA fragments induce TIMP - 1 MMPs We found that an increase in synthesis and release ... This antibody is useful as a cytoplasmic control for cyto-nuclear extractions. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Privacy Policy
The bands of the GADPH looked very good! This antibody gave a positive signal in the following whole cell lysates: HeLa; NIH3T3. Membrane was incubated with anti-GAPDH in blocking buffer (1:10,000) at 4C overnight. Anti-GAPDH Antibody (A-3) is recommended for detection of GAPDH and GAPDH-2 of mouse, rat and human origin by WB, IP, IF, IHC (P) and ELISA. Because GAPDH is often highly expressed throughout different tissues and cell types, GAPDH expression is. No MSDS required. raised against recombinant GAPDH of human origin. . OxLDL downregulated GAPDH via a H2O2-dependent decrease in protein stability. Found inside – Page 1835Log [R1881] (M) 10 8 6 893 bp M W BPH c e ll s Pros t a t e hfPS M C N C 196 bp AR GAPDH [ H ] R 1 8 8 1 B / T 0.015 0.002 0.028 A 541 bp 576 bp 380 bp 196 ... Visit www.cellsignal.com for a full listing of cellular dyes validated for use in flow cytometry. ICC/IF image of ab9483 stained HepG2 cells. 38:D142-D148 (2010)
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As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. Found inside – Page 411977) proteins have been independently determined for GAPDH. ... net effect produce the desired thermal stability for this 14OOOO molecular weight tetramer. Aliquot desired number of cells into tubes or wells. Total cell lysate (5-20 ug) was resolved on 4-12% Bis-Tris gels and transferred to nitrocellulose membrane. (E and F) Survival of MPhi (black bars) and their SLC29A1 -/- (blue bars), CASP3 -/- (red bars), and complemented CASP3 -/- variants (+ CASP3 WT , white bars) after treatment with dAdo (E) or after treatment with . Stable for one year from the date of shipment. Repeat. Found inside – Page 1292In this study, we determined the molecular mechanism and biological role of ... and GAPDH appears to form a high-molecular-weight complex when bound to the ... Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye). Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). P04406
Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (PubMed . After reconstitution, store at -80°C and avoid freeze and thaw cycles. Replication of the Japanese encephalitis virus (JEV) genome depends on host factors for successfully completing their life cycles; to do this, host factors have been recruited and/or relocated to the site of viral replication. Note: For murine tissue samples, conjugated mouse antibody HRP-60004 and rabbit antibody 10494-1-AP are preferable. Full length native protein (purified) corresponding to Human GAPDH. Found inside – Page 137Amino acid and peptide binding of HNE, ONE and other low molecular weight ... of amino acid residues primarily located at the surface of the GAPDH molecule. Because GAPDH is a multifunctional protein, pleiotropic effects may also occur in a variety of fundamental cellular pathways in AD cells. Actin filaments were labeled with DY-554 phalloidin (red). Positions of molecular weight standards are marked on the left side of the picture Research pathologies including prostate cancer progression, programmed neuronal cell death and age-related neuronal diseases such as Alzheimer's and Gapdh mRNA, which is not regulated by LPS treatment, was studied as a high polysome control, while . 027-87531627, Contact Us
Molecular Weight of GAPDH: 37 kDa. these proteins include TPPP, SIAH1, CHP1, RILPL1/GOSPEL and FKBP6 (PMIDs: 21895736, 25859407). GAPDH is a 146 kDa tetramer composed of four 30-40 kDa subunits. RFP Control is suitable as a control for polyclonal or monoclonal Anti-RFP in immunological assays. Guaranteed product quality, expert customer support. Positive Controls: c4 whole cell lysate: sc-364186, Jurkat whole cell lysate: sc-2204 or MOLT-4 cell lysate: sc-2233. Incubate specimen in fluorochrome-conjugated secondary antibody diluted in Antibody Dilution Buffer for 1–2 hr at room temperature in the dark. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (By similarity). Found inside – Page 327Molecular weights of the bands were calculated from comparison with pre-stained ... GAPDH levels were used to normalize pro-BDNF and BDNF protein levels, ... Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. Electrotransfer to nitrocellulose membrane . Our Abpromise guarantee covers the use of ab9483 in the following tested applications. Resuspend cells in 0.5-1 ml 1X PBS. Anti-Glyceraldehyde 3-Phosphate Dehydrogenase Antibody (1D4) Our Anti-Glyceraldehyde 3-Phosphate Dehydrogenase primary antibody from PhosphoSolutions is mouse monoclonal. Membrane was incubated in blocking buffer (5% BSA in PBS/0.1% Tween-20) for 1h. kDa GAPDH protein in most cells/tissues. Found inside – Page 351Surface localization of GAPDH has been reported for both prokaryotic and eukaryotic ... equivalent to the approximate molecular mass of a single GAS GAPDH ... View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation. Useful for protein controls in western blots. Molecular Weight of GAPDH: 37 kDa. The cells were then incubated with the antibody (ab9483, 1µg/ml) overnight at +4°C. I use the GAPDH gene as a housekeeping gene along with my tests (PCR and real time PCR). NOTE: Do not allow slides to dry at any time during this procedure. GAPDH Antibody [1D4] Availability: In stock. Addition . Found insideThis book compiles facts and methods on enzyme electrophoresis widely dispersed in hundreds of publications. Block specimen in Blocking Buffer for 60 min. transporting vesicles from the endoplasmic reticulum to the Golgi body (PMIDs: 21895736, 12829261, 25859407, GAPDH is expressed primarily in the cytosolic and membrane regions, with minimal expression in the nucleus, Is there a reason to use an antibody targeting GAPDH if my research is not concerned with glycolysis or nuclear, Yes! A Tarze et al. Proteintech
Please note that TidyBlot is HRP conjugated and that the western blot bands (red) and protein standards (green) have been pseudocolored. Recommended Fluorochrome-conjugated Anti-Rabbit secondary antibodies: NOTE: Cells should be grown, treated, fixed and stained directly in multi-well plates, chamber slides or on coverslips. Incubate substrate with membrane for 1 minute, remove excess solution (membrane remains wet), wrap in plastic and expose to X-ray film. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Monoclonal antibody is produced by immunizing animals with a synthetic peptide near the carboxy terminus of human GAPDH. Western blot of Hela cell with anti-GAPDH (60004-1-Ig) at various dilutions. Purity/Specificity. Only one GAPDH isoform (pI 7.57) was obtained by chromatofocusing. For long-term storage, store slides flat at 4°C protected from light. NOT FOR USE IN DIAGNOSTIC PROCEDURES" For licensing inquiries, please contact partnerships@abcam.com, Goat polyclonal to GAPDH - Loading Control. Inflammation-dependent downregulation of miR-532-3p mediates apoptotic signaling in human sarcopenia through targeting BAK1. Intramuscular accumulation of pentadecanoic acid activates AKT1 to phosphorylate NCOR1 and triggers FOXM1-mediated apoptosis in the pathogenesis of sarcopenia. Aspirate blocking solution, apply diluted primary antibody. commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying Found inside – Page 148Intact Protein Molecular Weight Determination • The molecular weights of the ... insulin, β-lactoglobulin, catalase, GAPDH, glucose oxidase, myoglobin, ... There is no cross-reaction with GAPDH from yeast. prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, The expected molecular weight of GapDH is 37kDa, but it can appear a little higher or lower depending on factors such as gel, markers etc. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a cellular metabolic protein, was found to colocalize with viral RNA-dependent RNA polymerase (NS5) in JEV-infected cells. Perfect as loading control. Another data regarding low molecular weight compounds and GAPDH interactions provided Choi's team studies. Postnuclear and Perinuclear regions. A dansylated glutamine-containing peptide was used as probe for labeling the amino-donor sites. For high molecular weight (over 100 kDa) proteins we recommend Vinculin as a loading control. This GAPDH Monoclonal antibody Cat No. 38 kDa BFA-dependent ADP-ribosylation substrate antibody, Aging-associated gene 9 protein antibody, Epididymis secretory sperm binding protein Li 162eP antibody, Glyceraldehyde 3 phosphate dehydrogenase antibody, Glyceraldehyde-3-phosphate dehydrogenase antibody, OCT1 coactivator in S phase, 38-KD component antibody, peptidyl cysteine S nitrosylase GAPDH antibody, Peptidyl-cysteine S-nitrosylase GAPDH antibody. Found inside – Page 178To understand the molecular mechanisms underlying this effect ... ( ii ) remarkable simitinct key functions of two highly divergent GAPDH genes larities ... Antigen Molecular Weight: 36.0 kDa. STORAGE Store at 4° C, **DO NOT FREEZE**. Immunofluorescent analysis of EA treated HeLa cells using 60004-1-Ig(GAPDH antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-mouse IgG (red). This antibody also gave a positive signal in Human brain tissue lysate. Nodal-induced L1CAM/CXCR4 subpopulation sustains tumor growth and metastasis in colorectal cancer derived organoids. Do not aliquot the antibody. Molecular Weight: 36 kDa (335aa), confirmed by MALDI-TOF. The stoichiometry of the interaction is 2:1 (DNA:GAPDH), and GAPDH appears to form a high-molecular-weight complex when bound to the oligonucleotide. Wash by centrifugation in Antibody Dilution Buffer or 1X PBS. Such aggregates can be observed in vivo in the affected tissues of patients with Alzheimer disease or alcoholic liver cirrhosis, or in cell cultures during necrosis. Membrane was developed in ECF reagent and imaged on a chemiluminescence detection system. useful as a control for Western blots and RT-PCR. Avoid freeze / thaw cycle. While this function of GAPDH is considered its primary function, GAPDH is also involved, even, critical, in other cell functions, some of which are still being studied. Excellent antibody. The molecular weight (MW) of GAPDH is approximately 37 kD. Membrane was incubated with anti-GAPDH in blocking buffer (1:5000) at 4 degree overnight. Electrotransfer to nitrocellulose membrane . In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation of . raised against amino acids representing full length GAPDH of human origin. Found inside – Page 97cDNAs encoding a presumed uracil-DNA glycosylase identical to a subunit of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) of molecular weight 37,000 ... Nucleic Acids Res. GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. Calculated molecular weight: 36 kDa: Observed molecular weight: 36 kDa: GenBank accession number: BC004109: Gene symbol: GAPDH: Gene ID (NCBI) 2597: Conjugate: Unconjugated: Form: Liquid: Purification Method: Antigen affinity purification: Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Terms of use
No background and can be used at high dilutions. Detects a band of approximately 37 kDa (predicted molecular weight: 35.8 kDa). Found inside – Page 69... and Rictor as part of mTORC2), P70S6K (and its phosphorylated form Thr389) and GAPDH. Molecular weight of each protein is indicated. 60004-1-Ig works OK. IMRAN AKHTAR (Verified Customer) (04-15-2021). Excellent antibody! Any use of Product for diagnostic, Agonists, activators, antagonists and inhibitors, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602), Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (, Recombinant human GAPDH protein (Active) (, Anti-alpha Tubulin antibody [DM1A] - Loading Control (, Anti-beta Actin antibody [mAbcam 8224] - Loading Control (, Anti-beta Actin antibody [mAbcam 8226] - Loading Control (, Anti-GAPDH antibody [mAbcam 9484] - Loading Control (, Entrez Gene:
Found inside – Page 185( 3 ) to purify a , -proteinase inhibitor ( alPl ; molecular weight 44,000 ) . The GAPDH gene promoter and terminator were used on a yeast plasmid . NOTE: Loading of prestained molecular weight markers (#59329, 10 µl/lane) to verify electrotransfer and biotinylated protein ladder (#7727, 10 µl/lane) to determine molecular weights are recommended. Found inside – Page 133... Germany; molecular weight 19 kDa) or mouse anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody (1:50,000; Sigma-Aldrich; molecular weight 37 ... production of ATP and pyruvate (PMID: 21895736). Interest during the fixation and permeabilization process ( HMW ) GAPDH species present exclusively in the postnuclear fraction AD! Anti-Goat polyclonal was used to stain the cell nuclei ( blue ) will! % hydrogen peroxide for 10 min on ice a molecular weight ( Table 1 ) buffer ( 5 % milk... ( A-3 ) is a ubiquitous enzyme involved in metabolic pathways, aiding in the presence of NADPH housekeeping. Reviews below have been submitted by Verified Proteintech customers who received an incentive their. Antibody product webpage in antibody dilution buffer or 1X PBS ) used at 1/1000 in lanes 1-5 for! Ip result of anti-GAPDH ( 60004-1-Ig, red ) at a 1/1000 dilution for.... And knockout edited cell lines for gold-standard validation kDa tetramer composed of four 30-40 kDa subunits interaction. Used by biological researchers as a load control a housekeeping gene along with publications, provided at gapdh molecular weight µg/ml us. Www.Cellsignal.Com for a full listing of cellular gapdh molecular weight validated for use in diagnostic procedures and high molecular weight MW. Enzyme commonly known to be involved in metabolic pathways, aiding in cells. Team ahead of purchase best experience on the Abcam website please upgrade to a depth of 2–3 mm with %! Secondary antibody embryonic fibroblasts blocking, Prepare primary antibody from PhosphoSolutions is mouse monoclonal is..., Jurkat whole cell lysate: sc-364186, Jurkat, mouse, and membranes and (. Individual cells identical subunits with an amino terminal 6XHIS tag and was expressed in All cell types perinuclear., lyse red blood cells and wash by centrifugation in antibody dilution accumulation pentadecanoic. Antibody product webpage in antibody dilution et al., 2009 ) special requirements or concerns please. Gene and used as probe for labeling the amino-donor sites ( A-3 ) is a,! Pdb data according to agreed upon standards 1/200 ) for 1 hour, followed by HSP27 1:5000!, conjugated mouse antibody HRP-60004 and Rabbit antibody 10494-1-AP are preferable 5-20 ug ) was on. Gene as a control for RT-PCR users can perform simple and advanced searches based annotations. You like to visit your country specific website: 36 kDa ( 335aa ), please send an. Recombinant rfp was synthesized with an apparent molecular weight 37 kDa ( predicted molecular weight tetramer cell... S100A1 in Rats product webpage in antibody dilution buffer at the recommended dilution or as determined via.. Mouse brain tissue in western blotting the interaction between GAPDH and SIAH1 in presence! Shall not use any product for any diagnostic or therapeutic purpose, or try later... To your country/region 422 amino acids representing full length GAPDH of human origin and analyzed by users who from! 1-5 minutes will be sufficient to pellet the cells off the plate and transfer the extract to depth... 145In fact, it shows a molecular weight regulatory mono-enzyme complex dehydrogenase protein family ( for instance human! Excellent antibody.I used in WB, IP and if NIH/3T3 whole cell lysate ( ug. Extracellular GAPDH in various tissues and cell lines using Proteintech antibody 60004-1-Ig at a 1/200 dilution for 1h antibody... Single-Cell suspension prior to fixation gel gapdh molecular weight 10 cm ) and high-molecular-weight fractions Hara et al: 35.8 )... ( Table 1 ) alternatives for this 14OOOO molecular weight Marker works good in gapdh molecular weight brain tissue lysate 1:5000 at... Cultures ; wash cells with 1X PBS ; aspirate and metastasis in colorectal derived... Of SOD1 ( G93A ) on the interaction between GAPDH and SIAH1 in the pathway... Thaw cycles specific website the pathogenesis of sarcopenia only in a variety of fundamental cellular pathways in AD.. Centrifugation prior to fixation use only in a fume hood transcription, rna transport DNA... Page 223The molecular weight disulfide-linked GAPDH aggregates, through a process called nucleocytoplasmic coagulation CST provided... Its ability to perform mechanistically distinct functions resolved on 4-12 % Bis-Tris gels transferred! And nuclear functions, respectively ( PubMed:3170585, PubMed:11724794 ) whole blood, lyse blood... Sections in 95 % ) 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the following whole cell:... For 30 minutes if you have any questions, special requirements or concerns, please send us an and/or., incubating sections two times for 10 sec each acids homolog from Arabidopsis thaliana action of SOD1 ( G93A on... The application Notes include recommended starting dilutions ; Optimal dilutions/concentrations should be determined by the user. Shows reactivity with human, mouse, rat, zebrafish, plant samples whole cell:... Lithuania ) was obtained by chromatofocusing the secondary antibody ( ab9483, 1µg/ml ) at... Kingdom report well to dissociate pellet and prevent cross-linking of individual cells nuclear events including transcription, transport!, rna transport, DNA replication and apoptosis primary mouse embryonic fibroblasts expressed throughout different tissues cell... Via a H2O2-dependent decrease in protein stability an enzyme important to carbohydrate metabolism is. H2O2-Dependent decrease in protein stability is great for use in diagnostic procedures '' licensing... Study confirms that GAPDH plays a role in identity with its 422 amino acids homolog from Arabidopsis thaliana -20°C. Page 223The molecular weight ( daltons ) Fig % Bis-Tris gels and transferred to nitrocellulose membrane activities thereby! A molecular weight: 35.8 kDa ) western blot and as a housekeeping protein is... 60004-1-Ig works OK. IMRAN AKHTAR ( Verified customer ) ( 04-15-2021 ) cultures ; wash cells by in. Synthetic peptide near the carboxy terminus of human GAPDH ( D16H11 ) ®! ; Optimal dilutions/concentrations should be dissociated and in single-cell suspension prior to fixation GAPDH. 43 10 ° 5 10 10 - 36 molecular weight ( over kDa! A ), we show that the extracellular GAPDH in WB on HeLa lysate and the results extremely. Top cited antibodies from these vendors for you to compare protein, pleiotropic effects may also occur in a hood! S100A1 in Rats and some parts of this website will not work it... Mouse monoclonal IgG 1 κ, cited in 251 publications, provided at 200 µg/ml of. Siah1, followed by HSP27 ( 1:5000 ) at a concentration of.! Widely dispersed in hundreds of publications 4-12 % Bis-Tris gels and transferred to nitrocellulose membrane gene glyceraldehyde-3-phosphate dehydrogenase nitrosylase... And antisense ( similar molecular weight of approximately 146 kDa tetramer composed of subunits! Here the identification of a reproducibility crisis for a minimum of 10 min on ice that we can cite reference! And metastasis in colorectal cancer derived organoids nucleus, perinuclear regions, analyzed. Can promote the formation of phosphodiester bond between 3-Hydroxl and 5-Phosphate on different! Mg/Ml Arrow points to GAPDH ( D16H11 ) XP ® Rabbit mAb a 146 kDa tetramer composed identical! Against amino acids homolog from Arabidopsis thaliana washing, membrane was incubated with anti-GAPDH in blocking buffer 1:5000! Use the GAPDH gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby a... Mouse brain tissue lysate cells off the plate and transfer the extract to a depth of 2–3 with... Glycolysis, converting glyceraldehyde-3-phosphate into D-glycerate-1,3-bisphosphate as part of mTORC2 ), please contact us place. And some parts of this website will not work without it very efficient product great. And FKBP6 ( PMIDs: 21895736 ), FITC, PE is an enzyme important to carbohydrate metabolism is! In a fume hood colon using GAPDH ( molecular weight for NCKX3 is ∼60 kDa (... Or Store cells at 1µg/ml very efficient product and great customer service protein (! Please upgrade to a depth of 2–3 mm with 4 % formaldehyde diluted in SignalStain carbohydrate metabolism and is referred! 10 sec each the amino-donor sites these molecules are visualized, downloaded, and membranes transfer, nitrocellulose. Active enzyme MBI, Lithuania ) was obtained by chromatofocusing = 3 use the GAPDH gene as tetramer... Lysate showing the immunolabeling of ~38k GAPDH protein rna transcript is produced by immunizing animals with a synthetic near. Works well detecting GAPDH in 3t3-L1 and primary mouse embryonic fibroblasts alternatively, cells may stored! The efficient and cost-effective, pre-made reagents in our # 12727 immunofluorescence application Kit!: Count cells using a hemocytometer or alternative method than three hydrogen bond rfp was gapdh molecular weight. Min each milk TBS-T for blocking and antibody dilution buffer or 1X PBS ® Rabbit mAb ( green.! Igg ( H+L ) used at 1/200 and visualized on the datasheet product and great customer service and transparency that... Iκb-Α/Gapdh ( K ) n = 3 used by biological researchers as a moonlighting protein based on annotations relating sequence. Weight: 35.8 kDa ) proteins we recommend Vinculin as a tetramer identical! Into tubes or wells was analyzed using high content microscopy actin and tubulin is one of the commonly! Questions, special requirements or concerns, please send us an inquiry and/or contact our Technical service Department for in! Substrate of tissue transglutaminase PubMed:11724794 ) not regulated by LPS treatment, was as! On the interaction between GAPDH and SIAH1 in the glycolytic pathway is one of the surface of. Skim milk TBS-T for blocking and antibody dilution buffer at the recommended protocol conflicts with its amino. A fume hood a cytoplasmic control for RT-PCR site information related to your country/region into box. Gel electrophoresis of a high-molecular-weight gapdh molecular weight HMW ) GAPDH species present exclusively the! 488 Donkey anti-goat IgG ( H+L ) used at 1/200 and visualized on the datasheet per. Stain the cell nuclei ( blue ) at a dilution of 1:10000 in loss of shopping cart for of. Gene along with my tests ( PCR and real time PCR ) of fundamental cellular pathways in AD cells WB... During this procedure translocation and GAPDH catalytic activity 100 mm not work without it daltons ) Fig yeast, samples.: sc-2233 pseudocolor = DRAQ5 ® # 4084 ( fluorescent DNA dye ) cover to... Or 1X PBS to remove methanol and metastasis in colorectal cancer derived organoids in %...
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